Ion-exchange Media

Ion-exchange (IEX) chromatography media is popularly employed to separate molecules, such as large proteins, small nucleotides and amino acids etc, based on their charge. The functional groups in IEX media have charges that bind molecules with opposite charges. Release of the bound molecules from the medium is achieved by displacement, via the application of an increasing concentration of a similarly charged molecule.

By design of the bead size, pore size, functional groups and the mechanical strength of individual base matrices, our IEX media can be used for capture of bulk product or impurities from large-volumes, as an intermediate purification step, or as a final step for high resolution purifications.

We offer an extensive range of ion exchange chromatography media (resins) in lab packs, bulk quantities and prepacked columns. Depending on the nature of your application, the property of your feedstock and the resolution you need, the general selection guide is shown along the product list below.

IEX Product Suitable Use
SepFastTM 6HF
General purpose preparative grade IEX media
Comparable to IEX Sepharose Fast Flow
SepFastTM Supor
(Q, S)
Polishing of antibodies
Purification of viruses, VLPs and plasmids etc
High capacity at high flow velocity
SepFastTM Capture
(Q, DEAE, S & CM)
High binding capacity
For capture step or intermediate purification step
Increased purification productivity
SepFastTM HighRes
(Q, DEAE, S & CM)
High resolution and high binding capacity
For intermediate and final purification steps, or for polishing step
SepFastTM Macro
(S, Q)
Purification of macro-molecules such as pegylated antibodies
High binding capacity and high resolution