Ion-exchange Media


Ion-exchange (IEX) chromatography media is popularly employed to separate molecules, such as large proteins, small nucleotides and amino acids etc, based on their charge. The functional groups in IEX media have charges that bind molecules with opposite charges. Release of the bound molecules from the medium is achieved by displacement, via the application of an increasing concentration of a similarly charged molecule.

By design of the bead size, pore size, functional groups and the mechanical strength of individual base matrices, our IEX media can be used for capture of bulk product or impurities from large-volumes, as an intermediate purification step, or as a final step for high resolution purifications.

We offer an extensive range of ion exchange chromatography media (resins) in lab packs, bulk quantities and prepacked columns. Depending on the nature of your application, the property of your feedstock and the resolution you need, the general selection guide is shown along the product list below.

IEX Product Suitable Use
Q, DEAE, SP & CM
SepFastTM 6HF

(50-150 m)
General purpose preparative grade IEX media
Comparable to IEX Sepharose Fast Flow
Inexpensive
Q, DEAE, SP & CM
SepFastTM 6HF Plus

(50-150 m)
Similar to IEX Sepharose FF XL, made of agarose and dextran
Increased binding capacity
Q, DEAE, SP & CM
SepFastTM Large Beads

(150-350 m)
General purpose preparative grade IEX media
Comparable to IEX Sepharose Big Beads
Particularly suitable for processing viscous or cell-containing feedstocks
Q, DEAE, SP & CM
SepFastTM Large Beads Plus

(150-350 m)
Large beads made of agarose and dextran
Increased binding capacity at faster flowrates
Q, DEAE, SP & CM
SepFastTM HighRes

(20-50 m)
Standard IEX media with reduced bead size to achieve better resolution
Inexpensive
Q, DEAE, SP & CM
SepFastTM HighRes Plus

(20-50 m)
Increased binding capacity
Increased resolution
SepFastTM
Supor Q, DEAE, S, CM
Polishing of antibodies
Purification of viruses, VLPs and plasmids etc
High capacity at high flow velocity
Magnetic SepFastTM
Supor Q, DEAE, S, CM
High-throughput screening of phage library for drug discovery

High-throughput purification of viruses
SepFastTM Macro
(Q, S)
Purification of macro-molecules such as pegylated antibodies
High binding capacity and high resolution