IEX SepFastTM Large Beads Plus Media
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Introduction
Q , DEAE , SP and CM SepFast Large Beads Plus are a group of ion exchange chromatography media with large particle sizes (100 – 350 μm) and high binding capacity. The base matrix shows excellent flow property and stable physical property.
The above ion-exchange chromatography media is particularly suitable for large-scale purification of biological molecules from viscous and/or crude culture broths or for high speed flow-through polishing applications in which impure components are chromatographically adsorbed from the main product stream.
This group of ion-exchange media has a balanced design among ligand density, loading capacity and separation power of individual components for large-scale biomanufacturing applications. IEX SepFast Large Beads Plus offers increased binding capacity and faster binding kinetics than IEX SepFast Large Beads.
The SepFast Large Beads Plus is made of highly cross-linked agarose grafted with dextran for increased binding capacity. They are very stable to most of the chemical conditions experienced in the bioprocessing industry.
Characteristics of IEX SepFast Large Beads Plus
Q SepFast Large Beads Plus | DEAE SepFast Large Beads Plus | SPSepFast Large Beads Plus | CM SepFast Large Beads Plus | |
Matrix | Highly cross-linked agarose and dextran | |||
Functionalgroup | Quaternary ammonium strong anion | Diethyl-aminoethyl weak anion | Sulfo strong cation | Carboxy- methyl weak cation |
Total ionic capacity | 0.1-0.2 mmol/ml | 0.1-0.2 mmol/ml | 0.1-0.2 mmol/ml | 0.1-0.2 mmol/ml |
Particle size | 100 – 350 μm | |||
Pressure-flow property* | >2000 cm/h | |||
Operational pressure | Up to 3 bar | |||
pH stability | 2-14 (short term) and 3-12 (long term) | |||
Working temperature | 4oC – 30oC | |||
Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol | |||
Total ionic capacity | Oxidizing agents, anionic detergents | Oxidizing agents, cationic detergents | ||
Storage | 20% ethanol | 20% ethanol | 20% ethanol + 0.2M sodium acetate | 20% ethanol |
*Measured in a 32 mm ID column at a bed height of 15 cm.
Ordering information
Description | Quantity | Code no. | Price GBP |
Q SepFast Large Beads Plus | 25 ml | 440201-25ML | £87.15 |
100 ml | 440201-100ML | £241.50 | |
1 Litre | 440201-1L | POR | |
5 Litre | 440201-5L | POR | |
50 Litre | 440201-50L | POR | |
DEAE SepFast Large Beads Plus | 25 ml | 440401-25ML | £87.15 |
100 ml | 440401-100ML | £241.50 | |
1 Litre | 440401-1L | POR | |
5 Litre | 440401-5L | POR | |
50 Litre | 440401-50L | POR | |
SP SepFast Large Beads Plus | 25 ml | 440601-25ML | £87.15 |
100 ml | 440601-100ML | £241.50 | |
1 Litre | 440601-1L | POR | |
5 Litre | 440601-5L | POR | |
50 Litre | 440601-50L | POR | |
CM SepFast Large Beads Plus | 25 ml | 440801-25ML | £87.15 |
100 ml | 440801-100ML | £241.50 | |
1 Litre | 440801-1L | POR | |
5 Litre | 440801-5L | POR | |
50 Litre | 440801-50L | POR |
*Other volume sizes are available on request.