IEX SepFastTM Large Beads Plus Media

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Introduction


Q , DEAE , SP and CM SepFast Large Beads Plus are a group of ion exchange chromatography media with large particle sizes (150 – 350 μm) and high binding capacity. The base matrix shows excellent flow property and stable physical property.

The above ion-exchange chromatography media is particularly suitable for large-scale purification of biological molecules from viscous and/or crude culture broths or for high speed flow-through polishing applications in which impure components are chromatographically adsorbed from the main product stream.

This group of ion-exchange media has a balanced design among ligand density, loading capacity and separation power of individual components for large-scale biomanufacturing applications. IEX SepFast Large Beads Plus offers increased binding capacity and faster binding kinetics than IEX SepFast Large Beads.

The SepFast Large Beads Plus is made of highly cross-linked agarose grafted with dextran for increased binding capacity. They are very stable to most of the chemical conditions experienced in the bioprocessing industry.

Characteristics of IEX SepFast Large Beads Plus

 
Q
SepFast Large Beads Plus
DEAE
SepFast Large Beads Plus
SP
SepFast Large Beads Plus
CM
SepFast Large Beads Plus
Matrix Highly cross-linked 6% agarose and dextran
Functional
group
Quaternary ammonium strong anion Diethyl-aminoethyl weak anion Sulfo strong cation Carboxy-
methyl weak cation
Total ionic capacity 0.1-0.2 mmol/ml 0.1-0.2 mmol/ml 0.1-0.2 mmol/ml 0.1-0.2 mmol/ml
Particle size 150 – 350 μm
Pressure-flow property* >2000 cm/h 
Operational pressure Up to 3 bar
pH stability 2-14 (short term) and 3-12 (long term)
Working temperature 4oC – 30oC
Chemical
stability
All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Total ionic capacity Oxidizing agents,
anionic detergents
Oxidizing agents,
cationic detergents
Storage 20% ethanol 20% ethanol 20% ethanol
+ 0.2M sodium acetate
20% ethanol

*Measured in a 32 mm ID column at a bed height of 15 cm.

Ordering information

Ordering information
Description Quantity Code no. Price GBP
Q SepFast Large Beads Plus 25 ml 440201-25ML 83.00
  100 ml 440201-100ML 230.00
  1 Litre 440201-1L POR
  5 Litre 440201-5L POR
  50 Litre 440201-50L POR
DEAE SepFast Large Beads Plus 25 ml 440401-25ML 83.00
  100 ml 440401-100ML 230.00
  1 Litre 440401-1L POR
  5 Litre 440401-5L POR
  50 Litre 440401-50L POR
SP SepFast Large Beads Plus 25 ml 440601-25ML 83.00
  100 ml 440601-100ML 230.00
  1 Litre 440601-1L POR
  5 Litre 440601-5L POR
  50 Litre 440601-50L POR
CM SepFast Large Beads Plus 25 ml 440801-25ML 83.00
  100 ml 440801-100ML 230.00
  1 Litre 440801-1L POR
  5 Litre 440801-5L POR
  50 Litre 440801-50L POR

*Other volume sizes are available on request.

How to order