IEX SepFastTM HighRes Plus Media
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Introduction
Q (DEAE, S, CM) SepFast™ HighRes Plus is a group of ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution and high binding capacity purification of biological molecules, where impure components are hard to separate by normal bioprocessing chromatography media.
The core advantages are:
- High binding capacity
- High resolution
- High sample loading capacity at high flow
- High productivity
IEX SepFast HighRes Plus is made of a composite of agarose and dextran to give increased dynamic binding capacities. The base matrix is highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.
Characteristics of IEX SepFast HighRes Plus
Q SepFast HighRes Plus | DEAE SepFast HighRes Plus | S SepFast HighRes Plus | CM SepFast HighRes Plus | |
Functionalgroup | Quaternary ammonium strong anion | Diethyl-aminoethyl weak anion | Sulfo strong cation | Carboxy- methyl weak cation |
Total ionic capacity | 0.09-0.15 mmol/ml | 0.08-0.13 mmol/ml | 0.09-0.15 mmol/ml | 0.08-0.13 mmol/ml |
Particle size | 20 – 50 μm | |||
Binding capacity* | >150 mg/ml BSA | >150 mg/ml BSA | >60 mg/ml hIgG | >150 mg/ml lysozyme |
Pressure-flow property* | To be run at a residence time of 2 min and above. | |||
Operational pressure | Up to 3 bar | |||
pH stability | 2-14 (short term) and 3-12 (long term) | |||
Working temperature | 4oC – 30oC | |||
Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol | |||
Total ionic capacity | Oxidizing agents, anionic detergents | Oxidizing agents, cationic detergents | ||
Storage | 20% ethanol | 20% ethanol | 20% ethanol + 0.2M sodium acetate | 20% ethanol |
*Measured in a 32 mm ID column at a bed height of 15 cm.
Ordering information
Description | Quantity | Code no. | Price GBP |
Q SepFast HighRes Plus | 25 ml | 470301-25ML | £120.75 |
100 ml | 470301-100ML | £330.75 | |
1 Litre | 470301-1L | POR | |
5 Litre | 470301-5L | POR | |
50 Litre | 470301-50L | POR | |
DEAE SepFast HighRes Plus | 25 ml | 470601-25ML | £120.75 |
100 ml | 470601-100ML | £330.75 | |
1 Litre | 470601-1L | POR | |
5 Litre | 470601-5L | POR | |
50 Litre | 470601-50L | POR | |
S SepFast HighRes Plus | 25 ml | 470901-25ML | £120.75 |
100 ml | 470901-100ML | £330.75 | |
1 Litre | 470901-1L | POR | |
5 Litre | 470901-5L | POR | |
50 Litre | 470901-50L | POR | |
CM SepFast HighRes Plus | 25 ml | 471201-25ML | £120.75 |
100 ml | 471201-100ML | £330.75 | |
1 Litre | 471201-1L | POR | |
5 Litre | 471201-5L | POR | |
50 Litre | 471201-50L | POR |
*Other volume sizes are available on request.