IEX SepFastTM Large Beads Media

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Introduction


Q , DEAE , SP and CM SepFast Large Beads are a group of ion exchange chromatography media with large particle sizes (150 – 350 μm). The base matrix shows excellent flow property and stable physical property.

The above ion-exchange chromatography media is particularly suitable for large-scale purification of biological molecules from viscous and/or crude culture broths or for high speed flow-through polishing applications in which impure components are chromatographically adsorbed from the main product stream.

This group of ion-exchange media has a balanced design among ligand density, loading capacity and separation power of individual components for large-scale biomanufacturing applications.

The base matrix of SepFast Large Beads is made of highly cross-linked agarose with excellent flow property. They are very stable to most of the chemical conditions experienced in the bioprocessing industry.

Characteristics of IEX SepFast Large Beads

 
Q
SepFast Large Beads
DEAE
SepFast Large Beads
SP
SepFast Large Beads
CM
SepFast Large Beads
Matrix Highly cross-linked 6% agarose
Functional
group
Quaternary ammonium strong anion Diethyl-aminoethyl weak anion Sulfo strong cation Carboxy-
methyl weak cation
Total ionic capacity 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml
Particle size 150 – 350 μm
Pressure-flow property* >2000 cm/h 
Operational pressure Up to 3 bar
pH stability 2-14 (short term) and 3-12 (long term)
Working temperature 4oC – 30oC
Chemical
stability
All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Total ionic capacity Oxidizing agents,
anionic detergents
Oxidizing agents,
cationic detergents
Storage 20% ethanol 20% ethanol 20% ethanol
+ 0.2M sodium acetate
20% ethanol

*Measured in a 32 mm ID column at a bed height of 15 cm.

Ordering information

Ordering information
Description Quantity Code no. Price GBP
Q SepFast Large Beads 25 ml 440101-25ML 48.00
  100 ml 440101-100ML 140.00
  1 Litre 440101-1L POR
  5 Litre 440101-5L POR
  50 Litre 440101-50L POR
DEAE SepFast Large Beads 25 ml 440301-25ML 48.00
  100 ml 440301-100ML 140.00
  1 Litre 440301-1L POR
  5 Litre 440301-5L POR
  50 Litre 440301-50L POR
SP SepFast Large Beads 25 ml 440501-25ML 48.00
  100 ml 440501-100ML 140.00
  1 Litre 440501-1L POR
  5 Litre 440501-5L POR
  50 Litre 440501-50L POR
CM SepFast Large Beads 25 ml 440701-25ML 48.00
  100 ml 440701-100ML 140.00
  1 Litre 440701-1L POR
  5 Litre 440701-5L POR
  50 Litre 440701-50L POR

*Other volume sizes are available on request.

How to order