IEX SepFastTM 6HF Plus Media
>>Introduction | >>Product Charactistics | >>Ordering Info | >>How to Order |
---|
Introduction
Q SepFast™ 6HF Plus, DEAE SepFast™ 6HF Plus, SP SepFast™ 6HF Plus and CM SepFast™ 6HF Plus are a group of strong anion exchange, weak anion exchange, strong cation exchange and weak cation exchange media, respectively.
The above ion-exchange chromatography (IEX) media is designed for the general purification of biological molecules where high binding capacity or faster binding kinetics is desirable.
The base matrix is made of highly cross-linked 6% agarose and dextran. It is very stable to most of the chemical conditions experienced in the bioprocessing industry. The IEX SepFast™ 6HF Plus could be a good alternative to the IEX Sepharose XL, with the benefit of reduced cost. We provide the regulatory support files of individual resins for those cGMP applications.
The core advantages to large-scale
biomanufacturings are:
- Much increased dynamic binding capacity
- Fast binding kinetics
- Affordable cost
Characteristics of IEX SepFast 6HF Plus
QSepFast 6HF Plus | DEAESepFast 6HF Plus | SPSepFast 6HF Plus | CMSepFast 6HF Plus | |
Matrix | Dextran and agarose composite | |||
Functionalgroup | Quaternary ammonium strong anion | Diethyl-aminoethyl weak anion | Sulfo strong cation | Carboxy- methyl weak cation |
Total ionic capacity | 0.09-0.15 mmol/ml | 0.08-0.13 mmol/ml | 0.09-0.15 mmol/ml | 0.08-0.13 mmol/ml |
Particle size | 50 – 150 μm | |||
Pressure-flow property* | >500 cm/h | |||
Operational pressure | Up to 3 bar | |||
pH stability | 2-14 (short term) and 3-12 (long term) | |||
Working temperature | 4oC – 30oC | |||
Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol | |||
Total ionic capacity | Oxidizing agents, anionic detergents | Oxidizing agents, cationic detergents | ||
Storage | 20% ethanol | 20% ethanol | 20% ethanol + 0.2M sodium acetate | 20% ethanol |
*Measured in a 32 mm ID column at a bed height of 15 cm.
Ordering information
Description | Quantity | Code no. | Price GBP |
Q SepFast 6HF Plus | 25 ml | 453001-25ML | £87.15 |
100 ml | 453001-100ML | £241.50 | |
1 Litre | 453001-1L | POR | |
5 Litre | 453001-5L | POR | |
50 Litre | 453001-50L | POR | |
DEAE SepFast 6HF Plus | 25 ml | 453101-25ML | £87.15 |
100 ml | 453101-100ML | £241.50 | |
1 Litre | 453101-1L | POR | |
5 Litre | 453101-5L | POR | |
50 Litre | 453101-50L | POR | |
SP SepFast 6HF Plus | 25 ml | 453201-25ML | £87.15 |
100 ml | 453201-100ML | £241.50 | |
1 Litre | 453201-1L | POR | |
5 Litre | 453201-5L | POR | |
50 Litre | 453201-50L | POR | |
CM SepFast 6HF Plus | 25 ml | 453301-25ML | £87.15 |
100 ml | 453301-100ML | £241.50 | |
1 Litre | 453301-1L | POR | |
5 Litre | 453301-5L | POR | |
50 Litre | 453301-50L | POR |
*Other volume sizes or pre-packed columns are available on request.