IEX SepFastTM 6HF Plus Media

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Introduction


Q SepFast™ 6HF Plus, DEAE SepFast™ 6HF Plus, SP SepFast™ 6HF Plus and CM SepFast™ 6HF Plus are a group of strong anion exchange, weak anion exchange, strong cation exchange and weak cation exchange media, respectively.

The above ion-exchange chromatography (IEX) media is designed for the general purification of biological molecules where high binding capacity or faster binding kinetics is desirable.

The base matrix is made of highly cross-linked 6% agarose and dextran. It is very stable to most of the chemical conditions experienced in the bioprocessing industry. The IEX SepFast™ 6HF Plus could be a good alternative to the IEX Sepharose XL, with the benefit of reduced cost. We provide the regulatory support files of individual resins for those cGMP applications.

 The core advantages to large-scale biomanufacturings are:

  • Much increased dynamic binding capacity
  • Fast binding kinetics
  • Affordable cost

Characteristics of IEX SepFast 6HF Plus

 
Q
SepFast 6HF Plus
DEAE
SepFast 6HF Plus
SP
SepFast 6HF Plus
CM
SepFast 6HF Plus
Matrix Dextran and agarose composite
Functional
group
Quaternary ammonium strong anion Diethyl-aminoethyl weak anion Sulfo strong cation Carboxy-
methyl weak cation
Total ionic capacity 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml
Particle size 50 – 150 μm
Pressure-flow property* >500 cm/h 
Operational pressure Up to 3 bar
pH stability 2-14 (short term) and 3-12 (long term)
Working temperature 4oC – 30oC
Chemical
stability
All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Total ionic capacity Oxidizing agents,
anionic detergents
Oxidizing agents,
cationic detergents
Storage 20% ethanol 20% ethanol 20% ethanol
+ 0.2M sodium acetate
20% ethanol

*Measured in a 32 mm ID column at a bed height of 15 cm.

Ordering information

Ordering information
Description Quantity Code no. Price GBP
Q SepFast 6HF Plus 25 ml 453001-25ML 83.00
  100 ml 453001-100ML 230.00
  1 Litre 453001-1L POR
  5 Litre 453001-5L POR
  50 Litre 453001-50L POR
DEAE SepFast 6HF Plus 25 ml 453101-25ML 83.00
  100 ml 453101-100ML 230.00
  1 Litre 453101-1L POR
  5 Litre 453101-5L POR
  50 Litre 453101-50L POR
SP SepFast 6HF Plus 25 ml 453201-25ML 83.00
  100 ml 453201-100ML 230.00
  1 Litre 453201-1L POR
  5 Litre 453201-5L POR
  50 Litre 453201-50L POR
CM SepFast 6HF Plus 25 ml 453301-25ML 83.00
  100 ml 453301-100ML 230.00
  1 Litre 453301-1L POR
  5 Litre 453301-5L POR
  50 Litre 453301-50L POR

*Other volume sizes are available on request.

How to order