Hydrophobic Interaction Chromatography (HIC) Media

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Introduction


SepFast Butyl, SepFast Pentyl, SepFast Hexyl, SepFast Phenyl, SepFast Heptyl and SepFast Octyl are hydrophobic interaction chromatography (HIC) adsorbents with carbon chains of C4, C5, C6, benzene ring, C7 and C8, respectively. They are specially designed for the purification of biological molecules based on their hydrophobicity profiles.

HIC is a versatile technique and could show high selectivity to individual molecules according to their exposed hydrophobic zones. It is particularly useful for intermediate and final-stage purifications. A HIC medium normally binds at moderate to high salt concentrations. It is therefore logical to place a HIC step after an IEX step where molecules are usually eluted at high salt conditions.

HIC media requires much milder purification conditions than reversed phase chromatography (RPC) media. Better biological activity could be maintained in HIC operations than RPC operations.

SepFast HIC media offers a broad range of choices from C4 to C8. Generally speaking, the longer the carbon chain, the higher the surface hydrophobicity. Process developers and manufacturers have the chance to choose the best HIC medium for their applications. The core advantages are:

  • High sample loading capacity
  • More choices than other suppliers
  • High separation power

The base matrix is made of agarose that has been highly cross-linked. It is very stable in most chemical conditions experienced in the bioprocessing industry.

For each type of HIC ligand, there is a choice of 2 different base matrices according to the pore size. The feature and selection guide is listed as follows:

Group 1 Group 2
SepFast Butyl 4HF SepFast Butyl 6HF
SepFast Pentyl 4HF SepFast Pentyl 6HF
SepFast Hexyl 4HF SepFast Hexyl 6HF
SepFast Phenyl 4HF SepFast Phenyl 6HF
SepFast Heptyl 4HF SepFast Heptyl 6HF
SepFast Octyl 4HF SepFast Octyl 6HF
The above HIC media is designed to purify most medium to large proteins. The above HIC media is designed to purify peptides or small to medium proteins.

Characteristics of SepFast HIC Media

 
Ligand Butyl Pentyl Hexyl Phenyl Heptyl Octyl
Matrix Highly cross-linked agarose beads; 4% agarose for SepFast 4HF and 6% agarose for SepFast 6HF.
Functional
group
-(CH2)3
CH3
-(CH2)4
CH3
-(CH2)5
CH3

-C6H6
-(CH2)6
CH3
-(CH2)7
CH3
Particle
size
50 – 150 μm
Pressure
-flow
property*
>500 cm/h for SepFast 6HF series; >300 cm/h for SepFast 4HF series
Operational
pressure
Up to 3 bar
pH
stability
2-14 (short term) and 3-12 (long term)
Working
temperature
4oC – 30oC
Chemical
stability
All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% acetonitrile, 30% isopropanol, 70% ethanol, 3 M (NH4)2SO4
Storage 20% ethanol

*Measured in a 32 mm ID column at a bed height of 15 cm.

Ordering information

 
SepFast Pentyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Heptyl Products SepFast Octyl Products
Butyl Products
Description Quantity Code no. Price GBP
SepFast Butyl 4HF 25 ml 480101 94.92
  100 ml 480102 250.01
  1 Litre 480103 POR
SepFast Butyl 4HF column 5 x 1 ml 480104 143.54
  1 x 5 ml 480105 103.01
  1 x 10 ml 480106 155.09
  1 x 20 ml 480107 248.85
SepFast Butyl 6HF 25 ml 480301 94.92
  100 ml 480302 250.01
  1 Litre 480303 POR
SepFast Butyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Heptyl Products SepFast Octyl Products
Pentyl Products
Description Quantity Code no. Price GBP
SepFast Pentyl 4HF 25 ml 480401 94.92
  100 ml 480402 250.01
  1 Litre 480403 POR
SepFast Pentyl 4HF column 5 x 1 ml 480404 143.54
  1 x 5 ml 480405 103.01
  1 x 10 ml 480406 155.09
  1 x 20 ml 480407 248.85
SepFast Pentyl 6HF 25 ml 480601 94.92
  100 ml 480602 250.01
  1 Litre 480603 POR
SepFast Butyl Products SepFast Pentyl Products SepFast Phenyl Products SepFast Heptyl Products SepFast Octyl Products
Hexyl Products
Description Quantity Code no. Price GBP
SepFast Hexyl 4HF 25 ml 480701 94.92
  100 ml 480702 250.01
  1 Litre 480703 POR
SepFast Hexyl 4HF column 5 x 1 ml 480704 143.54
  1 x 5 ml 480705 103.01
  1 x 10 ml 480706 155.09
  1 x 20 ml 480707 248.85
SepFast Hexyl 6HF 25 ml 480901 94.92
  100 ml 480902 250.01
  1 Litre 480903 POR
SepFast Butyl Products SepFast Pentyl Products SepFast Hexyl Products SepFast Heptyl Products SepFast Octyl Products
Phenyl Products
Description Quantity Code no. Price GBP
SepFast Phenyl 4HF 25 ml 481001 94.92
  100 ml 481002 250.01
  1 Litre 481003 POR
SepFast Phenyl 4HF column 5 x 1 ml 481004 143.54
  1 x 5 ml 481005 103.01
  1 x 10 ml 481006 155.09
  1 x 20 ml 481007 248.85
SepFast Phenyl 6HF 25 ml 481201 94.92
  100 ml 481202 250.01
  1 Litre 481203 POR
SepFast Butyl Products SepFast Pentyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Octyl Products
HeptylProducts
Description Quantity Code no. Price GBP
SepFast Heptyl 4HF 25 ml 481301 94.92
  100 ml 481302 250.01
  1 Litre 481303 POR
SepFast Heptyl 4HF column 5 x 1 ml 481304 143.54
  1 x 5 ml 481305 103.01
  1 x 10 ml 481306 155.09
  1 x 20 ml 481307 248.85
SepFast Heptyl 6HF 25 ml 481501 94.92
  100 ml 481502 250.01
  1 Litre 481503 POR
SepFast Butyl Products SepFast Pentyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Heptyl Products
Octyl Products
Description Quantity Code no. Price GBP
SepFast Octyl 4HF 25 ml 481601 94.92
  100 ml 481602 250.01
  1 Litre 481603 POR
SepFast Octyl 4HF column 5 x 1 ml 481604 143.54
  1 x 5 ml 481605 103.01
  1 x 10 ml 481606 155.09
  1 x 20 ml 481607 248.85
SepFast Octyl 6HF 25 ml 481801 94.92
  100 ml 481802 250.01
  1 Litre 481803 POR


>>Click here for SepFast Butyl Products

>>Click here for SepFast Pentyl Products

>>Click here for SepFast Hexyl Products

>>Click here for SepFast Phenyl Products

>>Click here for SepFast Octyl Products

>>Click here for SepFast Octyl Products

How to order

Data Sheet

Data sheet