Hydrophobic Interaction Chromatography (HIC) Media

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Introduction


SepFast Butyl, SepFast Pentyl, SepFast Hexyl, SepFast Phenyl, SepFast Heptyl and SepFast Octyl are hydrophobic interaction chromatography (HIC) adsorbents with carbon chains of C4, C5, C6, benzene ring, C7 and C8, respectively. They are specially designed for the purification of biological molecules based on their hydrophobicity profiles.

HIC is a versatile technique and could show high selectivity to individual molecules according to their exposed hydrophobic zones. It is particularly useful for intermediate and final-stage purifications. A HIC medium normally binds at moderate to high salt concentrations. It is therefore logical to place a HIC step after an IEX step where molecules are usually eluted at high salt conditions.

HIC media requires much milder purification conditions than reversed phase chromatography (RPC) media. Better biological activity could be maintained in HIC operations than RPC operations.

SepFast HIC media offers a broad range of choices from C4 to C8. Generally speaking, the longer the carbon chain, the higher the surface hydrophobicity. Process developers and manufacturers have the chance to choose the best HIC medium for their applications. The core advantages are:

  • High sample loading capacity
  • More choices than other suppliers
  • High separation power

The base matrix is made of agarose that has been highly cross-linked. It is very stable in most chemical conditions experienced in the bioprocessing industry.

For each type of HIC ligand, there is a choice of 2 different base matrices according to the pore size. The feature and selection guide is listed as follows:

Group 1 Group 2
SepFast Butyl 4HF SepFast Butyl 6HF
SepFast Pentyl 4HF SepFast Pentyl 6HF
SepFast Hexyl 4HF SepFast Hexyl 6HF
SepFast Phenyl 4HF SepFast Phenyl 6HF
SepFast Heptyl 4HF SepFast Heptyl 6HF
SepFast Octyl 4HF SepFast Octyl 6HF
The above HIC media is designed to purify most medium to large proteins. The above HIC media is designed to purify peptides or small to medium proteins.

Characteristics of SepFast HIC Media

 
Ligand Butyl Pentyl Hexyl Phenyl Heptyl Octyl
Matrix Highly cross-linked agarose beads; 4% agarose for SepFast 4HF and 6% agarose for SepFast 6HF.
Functional
group
-(CH2)3
CH3
-(CH2)4
CH3
-(CH2)5
CH3

-C6H6
-(CH2)6
CH3
-(CH2)7
CH3
Particle
size
50 – 150 μm
Pressure
-flow
property*
>500 cm/h for SepFast 6HF series; >300 cm/h for SepFast 4HF series
Operational
pressure
Up to 3 bar
pH
stability
2-14 (short term) and 3-12 (long term)
Working
temperature
4oC – 30oC
Chemical
stability
All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% acetonitrile, 30% isopropanol, 70% ethanol, 3 M (NH4)2SO4
Storage 20% ethanol

*Measured in a 32 mm ID column at a bed height of 15 cm.

Ordering information

 
SepFast Pentyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Heptyl Products SepFast Octyl Products
Butyl Products
Description Quantity Code no. Price GBP
SepFast Butyl 4HF 25 ml 480101 90.40
  100 ml 480102 238.10
  1 Litre 480103 POR
SepFast Butyl 4HF column 5 x 1 ml 480104 136.70
  1 x 5 ml 480105 98.10
  1 x 10 ml 480106 147.70
  1 x 20 ml 480107 237.00
SepFast Butyl 6HF 25 ml 480301 90.40
  100 ml 480302 238.10
  1 Litre 480303 POR
SepFast Butyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Heptyl Products SepFast Octyl Products
Pentyl Products
Description Quantity Code no. Price GBP
SepFast Pentyl 4HF 25 ml 480401 90.40
  100 ml 480402 238.10
  1 Litre 480403 POR
SepFast Pentyl 4HF column 5 x 1 ml 480404 136.70
  1 x 5 ml 480405 98.10
  1 x 10 ml 480406 147.70
  1 x 20 ml 480407 237.00
SepFast Pentyl 6HF 25 ml 480601 90.40
  100 ml 480602 238.10
  1 Litre 480603 POR
SepFast Butyl Products SepFast Pentyl Products SepFast Phenyl Products SepFast Heptyl Products SepFast Octyl Products
Hexyl Products
Description Quantity Code no. Price GBP
SepFast Hexyl 4HF 25 ml 480701 90.40
  100 ml 480702 238.10
  1 Litre 480703 POR
SepFast Hexyl 4HF column 5 x 1 ml 480704 136.70
  1 x 5 ml 480705 98.10
  1 x 10 ml 480706 147.70
  1 x 20 ml 480707 237.00
SepFast Hexyl 6HF 25 ml 480901 90.40
  100 ml 480902 238.10
  1 Litre 480903 POR
SepFast Butyl Products SepFast Pentyl Products SepFast Hexyl Products SepFast Heptyl Products SepFast Octyl Products
Phenyl Products
Description Quantity Code no. Price GBP
SepFast Phenyl 4HF 25 ml 481001 90.40
  100 ml 481002 238.10
  1 Litre 481003 POR
SepFast Phenyl 4HF column 5 x 1 ml 481004 136.70
  1 x 5 ml 481005 98.10
  1 x 10 ml 481006 147.70
  1 x 20 ml 481007 237.00
SepFast Phenyl 6HF 25 ml 481201 90.40
  100 ml 481202 238.10
  1 Litre 481203 POR
SepFast Butyl Products SepFast Pentyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Octyl Products
HeptylProducts
Description Quantity Code no. Price GBP
SepFast Heptyl 4HF 25 ml 481301 90.40
  100 ml 481302 238.10
  1 Litre 481303 POR
SepFast Heptyl 4HF column 5 x 1 ml 481304 136.70
  1 x 5 ml 481305 98.10
  1 x 10 ml 481306 147.70
  1 x 20 ml 481307 237.00
SepFast Heptyl 6HF 25 ml 481501 90.40
  100 ml 481502 238.10
  1 Litre 481503 POR
SepFast Butyl Products SepFast Pentyl Products SepFast Hexyl Products SepFast Phenyl Products SepFast Heptyl Products
Octyl Products
Description Quantity Code no. Price GBP
SepFast Octyl 4HF 25 ml 481601 90.40
  100 ml 481602 238.10
  1 Litre 481603 POR
SepFast Octyl 4HF column 5 x 1 ml 481604 136.70
  1 x 5 ml 481605 98.10
  1 x 10 ml 481606 147.70
  1 x 20 ml 481607 237.00
SepFast Octyl 6HF 25 ml 481801 90.40
  100 ml 481802 238.10
  1 Litre 481803 POR


>>Click here for SepFast Butyl Products

>>Click here for SepFast Pentyl Products

>>Click here for SepFast Hexyl Products

>>Click here for SepFast Phenyl Products

>>Click here for SepFast Octyl Products

>>Click here for SepFast Octyl Products

How to order

Data Sheet

Data sheet