SepFastTM Gel Filtration Media

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Introduction

SepFast gel filtration media is specially designed for high resolution and high recovery fractionation of biological molecules based on their molecular weights. Gel filtration (or called size exclusion chromatography) is a proven technique, which is widely used for size-based molecular separations.

SepFast gel filtration media has a balanced design to offer high recovery and high selectivity according to individual applications. The core advantages are:

  • High resolution
  • High selectivity
  • High recovery
  • Highly scalable
  • Excellent physical and mechanical stability
  • Cost effective

The base matrix is made of agarose or a composite of polysaccharides that have been highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.

The selection guide is listed as follows:

                               SepFast GF-HS
  SepFast GF-HS-M SepFast GF-HS-L
Separation range 3x103 - 7x104 6x103 - 6x105
Application Preparative grade separation for research, process development and large-scale productions
Particle Size(m)       20-50      20-50
Format supplied       Loose resin and pre-packed columns

Characteristics of  Gel Filtration Media

 
Matrix Highly cross-linked agarose and dextran composites
Particle size Around 35 m  (in the range of 20 – 50 m)
Operational pressure 3 bar (0.3 MPa, 42 psi) for SepFast GF-HS-L
4 bar (0.4 Mpa, 56 psi) for SepFast GF-HS-M
Operating flow velocity Normally 10-50 cm/hour
pH stability 2-14 (short term) and 3-12 (long term)
Working
temperature
4oC – 30oC
Chemical stability All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Avoid Oxidizing agents
Storage The media should be stored in 20% ethanol to prevent microbial growth. Store the media at a temperature of +2oC to +8oC.

Ordering information

 
Description Quantity Code no. Price GBP
SepFast GF-HS-M 25 ml 540201 109.20
  150 ml 540202 395.90
  1 Litre 540203 POR
  We also provide other bulk volumes. Please contact us for further information.
SepFast GF-HS-M column 16 x 600 mm 540204 1254.80
  26 x 600 mm 540205 POR
  We also provide pre-packed columns with other diameters and heights. Please contact us for further information
SepFast GF-HS-L 25 ml 540101 109.20
  150 ml 540102 395.90
  1 Litre 540103 POR
  We also provide other bulk volumes. Please contact us for further information.
SepFast GF-HS-L column 16 x 600 mm 540104 1195.00
  26 x 600 mm 540105 POR
  We also provide pre-packed columns with other diameters and heights. Please contact us for further information

How to order