Immobilised Metal Affinity Chromatography (IMAC) has been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. It is based on the specific interactions between certain transitional metal ions, mostly Ni2+, Zn2+ and Co2+ to the exposed amino acid surface chains containing histidine (or cysteine and tryptophane). The presence of several adjacent histidines such as (His)6-tag increases the affinity to immobilised metal ions. Increasingly, IMAC resins are employed for the purification of histidine-tagged recombinant proteins expressed in bacteria, yeast and mammalian cells. There are other applications of IMAC resins to purification of certain native non-tagged proteins as well, such as interferons, lectins, antibodies, serum and plasma proteins, peptides and peptide hormones.
We supply three types of IMAC resins. They are specifically designed and tailored to suit different protein purification activities (e.g. spin, gravity flow, packed bed etc). Therefore, you can choose the best type of IMAC resin with the benefit of great reduction in cost and time.
- IMAC SepFastTM
This type of resin has the best performance : price ratio for batch, gravity flow or packed column purifications.
- Pre-packed Ni SepFastTM
Ni SepFast resin is packed in our robust and durable columns. The available column sizes are 1 ml and 5 ml. Other pre-packed resins or column sizes are available on request. >>>more
- IMAC SuperSpinTM
This type of product is designed for the fast and cost-effective purification of small quantities of proteins using a centrifuge.
- Magnetic Resins
This type of resin is designed for magnetic protein purifications.