IEX SepFastTM 6HF Media

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Introduction


Q SepFast 6HF, DEAE SepFast 6HF, SP SepFast 6HF and CM SepFast 6HF are a group of strong anion exchange, weak anion exchange, strong cation exchange and weak cation exchange media, respectively.

The above ion-exchange chromatography (IEX) media is designed for the general purification of biological molecules in which impure components can be chromatographically separated from target molecules at a less demanding process. 

The base matrix is made of highly cross-linked 6% agarose. It is very stable to most of the chemical conditions experienced in the bioprocessing industry. The IEX SepFast 6HF could be a good alternative to the IEX Sepharose 6FF with the benefit of reduced cost. We provide the regulatory support files of individual resins for those cGMP applications.

 The core advantages to large-scale biomanufacturings are:

  • Good dynamic binding capacity
  • Good pressure / flow property
  • Low cost

Characteristics of IEX SepFast 6HF

 
Q
SepFast 6HF		 DEAE
SepFast 6HF		 SP
SepFast 6HF		 CM
SepFast 6HF
Matrix Highly cross-linked 6% agarose
Functional
group		 Quaternary ammonium strong anion Diethyl-aminoethyl weak anion Sulfo strong cation Carboxy-
methyl weak cation
Total ionic capacity 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml
Particle size 50 – 150 μm
Pressure-flow property* >500 cm/h 
Operational pressure Up to 3 bar
pH stability 2-14 (short term) and 3-12 (long term)
Working temperature 4oC – 30oC
Chemical
stability		 All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Total ionic capacity Oxidizing agents,
anionic detergents		 Oxidizing agents,
cationic detergents
Storage 20% ethanol 20% ethanol 20% ethanol
+ 0.2M sodium acetate		 20% ethanol

*Measured in a 32 mm ID column at a bed height of 15 cm.

Ordering information

Ordering information
Description Quantity Code no. Price GBP
Q SepFast 6HF 25 ml 452001-25ML £49.61
  100 ml 452001-100ML £145.53
  1 Litre 452001-1L POR
  5 Litre 452001-5L POR
  50 Litre 452001-50L POR
DEAE SepFast 6HF 25 ml 452101-25ML £49.61
  100 ml 452101-100ML £145.53
  1 Litre 452101-1L POR
  5 Litre 452101-5L POR
  50 Litre 452101-50L POR
SP SepFast 6HF 25 ml 452201-25ML £49.61
  100 ml 452201-100ML £145.53
  1 Litre 452201-1L POR
  5 Litre 452201-5L POR
  50 Litre 452201-50L POR
CM SepFast 6HF 25 ml 452301-25ML £49.61
  100 ml 452301-100ML £145.53
  1 Litre 452301-1L POR
  5 Litre 452301-5L POR
  50 Litre 452301-50L POR

*Other volume sizes or pre-packed columns are available on request.

How to order

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Data Sheet

Data sheet