IEX SepFastTM HighRes Media

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Introduction


Q (DEAE, S, CM) SepFast™ HighRes is a group of ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.

 The core advantages are:

  • High resolution at high loading
  • High sample loading capacity at high flow
  • High productivity

The base matrix of IEX SepFast HighRes is made of highly cross-linked agarose. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.

Characteristics of IEX SepFast HighRes

 
Q
SepFast HighRes		 DEAE
SepFast HighRes		 S
SepFast HighRes		 CM
SepFast HighRes
Functional
group		 Quaternary ammonium strong anion Diethyl-aminoethyl weak anion Sulfo strong cation Carboxy-
methyl weak cation
Total ionic capacity 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml 0.09-0.15 mmol/ml 0.08-0.13 mmol/ml
Particle size 20 – 50 μm
Binding capacity* >100 mg/ml BSA >100 mg/ml BSA >20 mg/ml hIgG >100 mg/ml lysozyme
Pressure-flow property* To be run at a residence time of 2 min and above.
Operational pressure Up to 3 bar
pH stability 2-14 (short term) and 3-12 (long term)
Working temperature 4oC – 30oC
Chemical
stability		 All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Total ionic capacity Oxidizing agents,
anionic detergents		 Oxidizing agents,
cationic detergents
Storage 20% ethanol 20% ethanol 20% ethanol
+ 0.2M sodium acetate		 20% ethanol

*Measured in a 32 mm ID column at a bed height of 15 cm.

Ordering information

Ordering information
Description Quantity Code no. Price GBP
Q SepFast HighRes 25 ml 470201-25ML £103.95
  100 ml 470201-100ML £278.25
  1 Litre 470201-1L POR
  5 Litre 470201-5L POR
  50 Litre 470201-50L POR
DEAE SepFast HighRes 25 ml 470501-25ML £103.95
  100 ml 470501-100ML £278.25
  1 Litre 470501-1L POR
  5 Litre 470501-5L POR
  50 Litre 470501-50L POR
S SepFast HighRes 25 ml 470801-25ML £103.95
  100 ml 470801-100ML £278.25
  1 Litre 470801-1L POR
  5 Litre 470801-5L POR
  50 Litre 470801-50L POR
CM SepFast HighRes 25 ml 471101-25ML £103.95
  100 ml 471101-100ML £278.25
  1 Litre 471101-1L POR
  5 Litre 471101-5L POR
  50 Litre 471101-50L POR

*Other volume sizes are available on request.

How to order

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Data Sheet

Data sheet