Protein A SuperSpin

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Introduction


Protein A SuperSpin is supplied for fast small-scale purification of immunoglobulins from various species (e.g. serum, ascites and mammalian cell culture broth etc). Protein A has been well proven a powerful affinity ligand which shows very high specificity to immunoglobulins from mammalian hosts.

Each Protein A SuperSpin tube accommodates 50 l of Protein A SepFast particles. The affinity resin is specifically designed to provide large pore size with small particle size (i.e. 30 – 100 m). Thus large antibody molecules can access the affinity ligand rapidly with excellent mass transfer rate. As a result, half milligram quantities of antibody can be easily purified within 20 minutes at low cost. No special expensive chromatography instrument or column is required.

    Protein A SuperSpin is particularly useful for the following applications:
  • The quantity of antibody to be purified is small (less than half a milligram)
  • On-line detection of antibody expression level
  • High throughput purification of antibodies
  • High throughput screening of antibodies
    Key benefits:
  • Rapid antibody capture and recovery
  • High antibody binding capacity (half milligram)
  • High throughput format (24 samples can be processed in parallel)
  • Low cost
  • Easy to use and no expensive equipment required

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Characteristics of Protein A SuperSpin

 
Tube material Polypropylene
Resin Protein A SepFast
Particle size 30 – 100 m
Packed volume 50 l
Protein binding capacity Depends on the type of proteins and binding conditions;
up to 500 g
Chemical compatibility  The resin maintains the binding capability after exposing to 3M NaSCN, 6M GuHCl or 8M urea over a few hours
Storage condition 4oC – 30oC

Ordering information

Ordering information
Product Quantity Code no. Price GBP
Protein A SuperSpin 50 230102 250.95
Ordering information
Related products Quantity Code no. Price GBP
SuperSpin Desaltor 50 210101 154.56

How to order

Protein A SuperSpin

Operation Manual

Operation Manual