Protein G SepFast Range
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Protein G SepFast is a family of affinity resins with protein G immobilized to highly cross-linked agarose-based beads of various sizes.
- Protein G SepFast HighRes: 20 – 50 µm, good for small column run at shorter residence time (i.e. higher flowrate)
- Protein G SepFast: 50 – 150 µm, good for most applications from small scale to large scale purification requirements (i.e. low column pressure);
- Protein G SepFast Large Bead: 150 – 350 µm, particularly good for processing viscous or cell-containing feedstocks (i.e. high settling rate and extremely low column pressure)
Protein G binds to the Fc region of IgG from a variety of mammalian species. Protein G SepFast may be used to isolate and purify classes, subclasses and fragments of immunoglobins from any biological fluid or cell culture medium. Protein G SepFast is extremely useful for isolation of immune complexes.
The potential applications of protein G include practically all of the current and projected applications of protein A. Protein G and protein A, however, have different IgG binding specificities, dependent on the origin of the IgG. Compared to protein A, protein G binds more strongly to polyclonal IgG, for example, from cow, sheep and horse. Furthermore, unlike protein A, protein G binds polyclonal rat IgG, human IgG3 and mouse IgG1.
Protein G SepFast is developed and supported for production scale chromatography. BioToolomics offers both loose medium and pre-packed ready-to-use disposable columns.
Characteristics of Protein G SepFast Range
|Matrix||Highly cross-linked agarose bead|
|Particle size||20 - 50 µm Protein G SepFast HighRes50 - 150 µm Protein G SepFast 150 - 350 µm Protein G SepFast Large Bead|
|Max. operating pressure||0.3 MPa (3 bar, 42 psi)|
|Recommended flow rate*||50 to 300 cm/h|
|Binding capacity**||≥ 15 mg human IgG/mL resin|
|Chemical compatibility||The commonly used reagents for antibody purifications|
|Chemical stability||Stable in most commonly used buffers, 6 M guanidine-HCl; 70% ethanol, 8 M urea, pH 10.5; 0.1 M Glycine-NaOH, pH 11.|
|Physical stability||Negligible volume variation due to changes in pH or ionic strength.|
|pH stability||3 to 9 (long term); 2 to 10 (short term)|
|Sanitization||Sanitize the column with 70% ethanol|
|Storage & temperature||20% ethanol at 2oC – 8oC|
|Product||Quantity||Code no.||Price GBP|
|Protein G SepFast HighRes||5 ml||230701-5ML||£410.66|
|1 ml HiSep column||230702-1ML||£96.60|
|5 ml HiSep column*||230702-5ML||£446.78|
|Protein G SepFast||5 ml||230501-5ML||£358.16|
|1 ml HiSep column||230502-1ML||£86.10|
|5 ml HiSep column*||230502-5ML||£415.28|
|Protein G SepFast Large Bead||5 ml||230601-5ML||£358.16|
*Other column size available on request.