Protein G SepFast Range

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Protein G SepFast is a family of affinity resins with protein G immobilized to highly cross-linked agarose-based beads of various sizes.

  • Protein G SepFast HighRes: 20 – 50 m, good for small column run at shorter residence time (i.e. higher flowrate)
  • Protein G SepFast: 50 – 150 m, good for most applications from small scale to large scale purification requirements (i.e. low column pressure);
  • Protein G SepFast Large Bead: 150 – 350 m, particularly good for processing viscous or cell-containing feedstocks (i.e. high settling rate and extremely low column pressure)

Protein G binds to the Fc region of IgG from a variety of mammalian species. Protein G SepFast may be used to isolate and purify classes, subclasses and fragments of immunoglobins from any biological fluid or cell culture medium. Protein G SepFast is extremely useful for isolation of immune complexes.

The potential applications of protein G include practically all of the current and projected applications of protein A. Protein G and protein A, however, have different IgG binding specificities, dependent on the origin of the IgG. Compared to protein A, protein G binds more strongly to polyclonal IgG, for example, from cow, sheep and horse. Furthermore, unlike protein A, protein G binds polyclonal rat IgG, human IgG3 and mouse IgG1.

Protein G SepFast is developed and supported for production scale chromatography. BioToolomics offers both loose medium and pre-packed ready-to-use disposable columns.

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Characteristics of Protein G SepFast Range

*Linear flow rate = volumetric flow rate (cm3/h)/column cross-sectional area (cm2);
**Please note that there might be considerable deviations in binding capacity for different immunoglobulins derived from the same species, even if they are of the same subclass.

Matrix Highly cross-linked agarose bead
Particle size 20 - 50 m Protein G SepFast HighRes
50 - 150 m Protein G SepFast
150 - 350 m Protein G SepFast Large Bead
Max. operating pressure 0.3 MPa (3 bar, 42 psi)
flow rate*
50 to 300 cm/h
Binding capacity** ≥ 15 mg human IgG/mL resin
Chemical compatibility The commonly used reagents for antibody purifications
Chemical stability Stable in most commonly used buffers, 6 M guanidine-HCl; 70% ethanol, 8 M urea, pH 10.5; 0.1 M Glycine-NaOH, pH 11.
Physical stability Negligible volume variation due to changes in pH or ionic strength.
pH stability 3 to 9 (long term); 2 to 10 (short term)
Sanitization Sanitize the column with 70% ethanol
Storage & temperature 20% ethanol at 2oC – 8oC

Ordering information

Ordering information
Product Quantity Code no. Price GBP
Protein G SepFast HighRes 5 ml 230701-5ML 410.66
25 ml 230701-25ML 1571.43
100 ml 230701-100ML POR
250 ml 230701-250ML POR
500 ml 230701-500ML POR
1 litre 230701-1L POR
1 ml HiSep column 230702-1ML 96.60
5 ml HiSep column* 230702-5ML 446.78
Protein G SepFast 5 ml 230501-5ML 358.16
25 ml 230501-25ML 1424.43
100 ml 230501-100ML POR
250 ml 230501-250ML POR
500 ml 230501-500ML POR
1 litre 230501-1L POR
1 ml HiSep column 230502-1ML 86.10
5 ml HiSep column* 230502-5ML 415.28
Protein G SepFast Large Bead 5 ml 230601-5ML 358.16
25 ml 230601-25ML 1424.43
100 ml 230601-100ML POR
250 ml 230601-250ML POR
500 ml 230601-500ML POR
1 litre 230601-1L POR

*Other column size available on request.

How to order