Heparin SepFast Media

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Heparin SepFast is an affinity chromatography medium that is used for the purification of biomolecules that show affinity to heparin, such as antithrombin III, coagulation factors and other plasma proteins, DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.

The base matrix is made of cross-linked porous agarose. The particles have an open pore structure with good mass transfer properties to large protein molecules. Heparin is immobilized to the base matrix by reducing amination method.

The medium shows high mechanical rigidity, so it can be operated at high flow velocities with moderate pressure drops. The medium is compatible with most of the chemical reagents commonly used in biological systems.

Characteristics of  Heparin SepFast Media

Matrix Agarose
Functional group Heparin of porcine origin
Ligand density 4 - 5 mg/ml
Particle size 50 - 150 m
pH stability 4-13 (short term) and 4-12 (long term)
4oC – 30oC
Chemical stability All commonly used buffers, 6M guanidine-HCl, 8 M urea
Storage 0.05 M sodium acetate in 20% denatured ethanol

Ordering information

Description Quantity Code no. Price GBP
Heparin SepFast 25 ml 160101-25ML 226.13
  100 ml 160101-100ML 639.35
  1 Litre 160101-1L POR

How to order

Operation Manual

Operation Manual