Protein A & Alkaline-stable Protein A SepFast Range

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Protein A SepFast is a family of affinity chromatography media for the purification of immunoglobulins. Its purification power has been well documented in various antibody purification applications, such as isolation and purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.

  • Protein A SepFast HighRes: 20 – 50 m, good for small column run at shorter residence time (i.e. higher flowrate);
  • Protein A SepFast HighRes Endure: 20 – 50 m, more alkaline-stable than Protein A SepFast HighRes, can be cleaned with 0.5 M NaOH;
  • Protein A SepFast: 50 – 150 m, good for most applications from small scale to large scale purification requirements (i.e. low column pressure);
  • Protein A SepFast Endure: 50 – 150 m, more alkaline-stable than Protein A SepFast, can be cleaned with 0.5 M NaOH;
  • Protein A SepFast Large Bead: 150 – 350 m, particularly good for processing viscous or cell-containing feedstocks (i.e. high settling rate and extremely low column pressure);
  • Protein A SepFast Large Bead Endure: 150 – 350 m, more alkaline-stable than Protein A SepFast Large Bead, can be cleaned with 0.5 M NaOH;

Standard protein A molecule or more alkaline-stable protein A is immobilised to highly porous and highly cross-linked agarose base matrices. Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. The medium shows high mechanical rigidity, so it can be operated at moderate to high flow velocities with moderate pressure drop.

BioToolomics offers both loose media and pre-packed ready-to-use disposable columns for small scale sample preparation and large-scale bioprocessing use.

The range of Protein A SepFast media is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.

>>>More info for Protein A Range

>>>More info for Protein A Endure Range

Characteristics of Protein A SepFast Range & Protein A SepFast Endure Range

Matrix Highly cross-linked agarose bead
Particle size 20 - 50 m Protein A SepFast HighRes or HighRes Endure
50 - 150 m Protein A SepFast or SepFast Endure 
150 - 350 m Protein A SepFast Large Bead or Large Bead Endure
Max. operating pressure 0.3 MPa (3 bar, 42 psi)
flow rate*
50 to 300 cm/h
Binding capacity** ≥ 30 mg human IgG/mL resin
Chemical compatibility The commonly used reagents for antibody purifications
Chemical stability Stable in most commonly used buffers, 6 M guanidine-HCl; 70% ethanol, 8 M urea, pH 10.5; 0.1 M Glycine-NaOH, pH 11.
Physical stability Negligible volume variation due to changes in pH or ionic strength.
pH stability 3 to 9 (long term); 2 to 10 (short term)
Sanitization Protein A SepFast Endure can be sanitised with 0.5 M NaOH
Storage & temperature 20% ethanol at 2oC – 8oC

*Linear flow rate = volumetric flow rate (cm3/h)/column cross-sectional area (cm2)
** Please note that there might be considerable deviations in binding capacity for different immunoglobulins derived from the same species, even if they are of the same subclass.

Ordering information

Ordering information
Product Quantity Code no. Price GBP
Protein A SepFast HighRes 5 ml 230801-5ML 410.66
25 ml 230801-25ML 1571.43
100 ml 230801-100ML POR
250 ml 230801-250ML POR
500 ml 230801-500ML POR
1 litre 230801-1L POR
1 ml HiSep column 230802-1ML 96.60
5 ml HiSep column* 230802-5ML 446.78
Protein A SepFast HighRes Endure 5 ml 230901-5ML 557.66
25 ml 230901-25ML 2379.93
100 ml 230901-100ML POR
250 ml 230901-250ML POR
500 ml 230901-500ML POR
1 litre 230901-1L POR
1 ml HiSep column 230902-1ML 128.10
5 ml HiSep column* 230902-5ML 614.78
Protein A SepFast 5 ml 230101-5ML 358.16
25 ml 230101-25ML 1424.43
100 ml 230101-100ML POR
250 ml 230101-250ML POR
500 ml 230101-500ML POR
1 L 230101-1L POR
5 L 230101-5L POR
10 L 230101-10L POR
1 ml HiSep column 230102-1ML 86.10
5 ml HiSep column* 230102-5ML 415.28
Protein A SepFast Endure 5 ml 230301-5ML 526.16
25 ml 230301-25ML 2169.93
100 ml 230301-100ML POR
250 ml 230301-250ML POR
500 ml 230301-500ML POR
1 L 230301-1L POR
5 L 230301-5L POR
10 L 230301-10L POR
1 ml HiSep column 230302-1ML 107.10
5 ml HiSep column* 230302-5ML 572.78
Protein A SepFast Large Bead 5 ml 230201-5ML 358.16
25 ml 230201-25ML 1424.43
100 ml 230201-100ML POR
250 ml 230201-250ML POR
500 ml 230201-500ML POR
1 L 230201-1L POR
5 L 230201-5L POR
10 L 230201-10L POR
Protein A SepFast Large Bead Endure 5 ml 230401-5ML 526.16
25 ml 230401-25ML 2169.93
100 ml 230401-100ML POR
250 ml 230401-250ML POR
500 ml 230401-500ML POR
1 L 230401-1L POR
5 L 230401-5L POR
10 L 230401-10L POR

*Other column size available on request.

How to order