5 ml and 10 ml Column

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Our 5 ml and 10 ml column has a column diameter of 11 mm. They are low cost but high performance empty disposable chromatography columns.

Traditional glass columns are too expensive that people can’t afford a large inventory. A user has to pack, unpack and repack the same column for different purification tasks. In particular, it could be very inconvenient if a laboratory needs to purify a good number of different molecules.

BioToolomics range of disposable chromatography columns has all the functionalities of small glass columns but possesses many other advantages: low cost, flexible, resin compressibility taken into account, disposable, easy to pack, tight packing of any type of chromatography media (>20 micrometer), identical design to the scale-up columns, robust, precise and reproducible packing, allowing moderate back pressure, easy to store, long life time.

After the chosen resin is packed in the column, the column can be readily stored and used for many times during the life time of the packed resin. Due to its low cost, a user can afford to assign individual columns for the purification of individual molecules.

The column body (i.e. the tube) is made of acrylic. It has the appearance similar to glass tube, i.e. clear and transparent.

The End Plug is made of polypropylene. It has the standard connection (10.32 UNF or called 1/16" female thread) compatible to common chromatography instruments (such as AKTA etc). It has two layers of mesh (coarse and fine) giving excellent flow distribution. The void volume in each End Plug is minimal as the fluid is introduced through a narrow flow path (i.e. 1 mm hole). Two O-rings are used in each End Plug to seal and secure the column.

The column material shows excellent chemical resistance to most of the commonly used reagents. The recommended operational pressure is up to 3 bar (42 psi).

Ordering information

Product* Quantity** Code no. Price GBP
SepFast column – 5 ml 1 unit 240107 34.10
SepFast column – 10 ml 1 unit 240108 41.80
Stop Plug 10 / pack 240104 45.11
10.32 male thread / female luer connector 5 / pack 240105 45.11

*For columns of other volumes or higher pressure ratings, please contact us for a quotation.

**The minimum order is 5 columns. If large quantity is required, please contact us for a quotation. BioToolomics also provides service to pack customers’ media of their choice.

How to order


Operation Manual

Operation Manual