SepFastTM Supor Q
>>Introduction | >>Ordering Info | >>How to Order |
---|
Introduction
SepFastTM Supor Q is a strong anion exchange chromatography
product in a ready-to-use disposable format. The working medium possesses a
combination of small pores (50-100nm) and large pores (micro level). It shows fast
accessibility to both small and large molecules (e.g. plasmid and virus).
Therefore, it has comparable binding capacity to those of membrane or monolith
product in respect of DNA or virus. However, SepFastTM Supor Q shows much higher
binding capacity to protein molecules than the membrane or monolith type of
products.
The base matrix is made of a composite of polysaccharides that have been well
cross-linked. The medium is stable to most of the chemical conditions
experienced in the bioprocessing industry.
It shows excellent binding capacity to both protein and macromolecule e.g. DNAs
(see the Table 1) at high flowrate. Certainly the capacity is further increased
at lower flowrates.
Table 1: A comparison of the dynamic binding capacity (DBC at 10% breakthrough) of different products (1 cm bed height):
SepFastTM Supor Q 10 CV |
Product A
(particle format) 10 CV |
Product A (particle format) 5 CV |
Product B (particle format) 5 CV |
|
Calf thymus DNA | 7075 µg/ml | 105 µg/ml | 1367µg/ml | 157 µg/ml |
Protein (BSA) | 133 mg/ml | 84mg/ml | 116 mg/ml | 1.7 mg/ml |
SepFastTM Supor Q can be used in the final polishing step
(shallow bed format) or be used for the binding-elution of both small (proteins)
and large molecules (e.g. plasmid and virus) at deep bed format. Please contact
us for further information.
It can be operated at both high flowrate or low-to-moderate flowrate. The column
is compatible to most of the liquid-handling equipment. The operational pressure
should not exceed 4 bars.
The medium was stored in 20% denatured ethanol. Before a chromatography run,
equilibrate the column with working buffer until the effluent shows stable
conductivity and pH value.
We recommend scouting for optimal binding pH and for optimal ionic strength.
SepFastTM Supor Q has a protein binding capacity (tested with BSA) virtually
independent of the flowrate. We recommend to pay special attention to optimising
elution conditions to avoid tailing in the elution step.
Ordering information
Product | Quantity* | Code no. | Price GBP |
SepFastTM Supor Q, 5 ml | 5 columns | 260103 | £440.27 |
SepFastTM Supor Q, 1 ml | 5 columns | 260104 | £133.46 |
*Other volume sizes are available on request.
How to order
SepFast Supor Q