SepFastTM Capture IEX Media

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Introduction


SepFast Capture Q, SepFast Capture DEAE, SepFast Capture S and SepFast Capture CM are, respectively, strong anion, weak anion, strong cation and weak cation exchange adsorbents. They are specially designed for cost-effective large-scale capturing of biological molecules in the initial downstream recovery step.

SepFast Capture media has a balanced design between mechanical strength and mass transfer property of individual particles. The core advantages to large-scale biomanufacturings are:

  • Very high dynamic binding capacity at high flow
  • High productivity
  • Reduced cost

The base matrix is made of a composite of polysaccharides that have been highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry. For each type of ion exchange medium, there is a choice of three different base matrices according to pore accessibility of target molecules. The feature and selection guide is listed as follows:

400 serial 500 serial 600 serial
SepFast Capture
Q-400		 SepFast Capture
Q-500		 SepFast Capture
Q-600
SepFast Capture
DEAE-400		 SepFast Capture
DEAE-500		 SepFast Capture
DEAE-600
SepFast Capture
S-400		 SepFast Capture
S-500		 SepFast Capture
S-600
SepFast Capture
CM-400		 SepFast Capture
CM-500		 SepFast Capture
CM-600
The above media is designed to capture smaller proteins or peptides (e.g. M.W. <100K Dalton). The above media is designed to capture antibodies or other molecules of similar size. The above media is designed to capture large proteins, plasmids or viral particles.

Characteristics of SepFast Capture IEX Media

 
Capture Q-400
Capture Q-500
Capture Q-600		 Capture DEAE-400
Capture DEAE-500
Capture DEAE-600		 Capture S-400
Capture S-500
Capture S-600		 Capture CM-400
Capture CM-500
Capture CM-600
Matrix Highly cross-linked polysaccharide composites
Functional group Quaternary ammonium strong anion Diethyl-aminoethyl weak anion Sulfo strong cation Carboxy-
methyl weak cation
Total ionic capacity 0.04-0.18 mmol/ml 0.04-0.18 mmol/ml 0.04-0.18 mmol/ml 0.04-0.18 mmol/ml
Particle size 50 – 150 μm
Dynamic binding capacity* >150 mg/ml BSA at 1 min residence time for Capture Q-400 >150 mg/ml BSA at 1 min residence time for Capture DEAE-400 >100 mg/ml hIgG at 1 min residence time for Capture S-500 >150 mg/ml lysozyme at 1 min residence time for Capture CM-400
Pressure-flow property** >1000 cm/h for Q-400, DEAE-400, S-400 and CM-400;
>500 cm/h for Q-500, DEAE-500, S-500 and CM-500;
>300 cm/h for Q-600, DEAE-600, S-600 and CM-600
Operational pressure Up to 3 bar
pH stability 2-14 (short term) and 3-12 (long term)
Working temperature 4oC – 30oC
Chemical stability All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol
Total ionic capacity Oxidizing agents, anionic detergents Oxidizing agents, cationic detergents
Storage 20% ethanol 20% ethanol 20% ethanol + 0.2 M sodium acetate 20% ethanol

*Measured at a breakthrough of 10%.
**Measured in a 32 mm ID column at a bed height of 20 cm.

Ordering information

 
SepFast Capture DEAE Products SepFast Capture S Products SepFast Capture CM Products
Capture Q Products
Description Quantity Code no. Price GBP
SepFast Capture Q-400 25 ml 450101 £71.72
  100 ml 450102 £199.61
  1 Litre 450103 POR
Disposable SepFast Capture Q-400 column 5 x 1 ml 450104 £111.41
  1 x 5 ml 450105 £81.59
  1 x 10 ml 450106 £108.05
  1 x 20 ml 450107 £184.17
SepFast Capture Q-500 25 ml 450201 £71.72
  100 ml 450202 £199.61
  1 Litre 450203 POR
Disposable SepFast Capture Q-500 column 5 x 1 ml 450204 £111.41
  1 x 5 ml 450205 £81.59
  1 x 10 ml 450206 £108.05
  1 x 20 ml 450207 £184.17
SepFast Capture Q-600 25 ml 450301 £71.72
  100 ml 450302 £199.61
  1 Litre 450303 POR
Disposable SepFast Capture Q-600 column 5 x 1 ml 450304 £111.41
  1 x 5 ml 450305 £81.59
  1 x 10 ml 450306 £108.05
  1 x 20 ml 450307 £184.17
SepFast Capture Q Products SepFast Capture S Products SepFast Capture CM Products
Capture DEAE Products
Description Quantity Code no. Price GBP
SepFast Capture DEAE-400 25 ml 450401 £71.72
  100 ml 450402 £199.61
  1 Litre 450403 POR
Disposable SepFast Capture DEAE-400 column 5 x 1 ml 450404 £111.41
  1 x 5 ml 450405 £81.59
  1 x 10 ml 450406 £108.05
  1 x 20 ml 450407 £184.17
SepFast Capture DEAE-500 25 ml 450501 £71.72
  100 ml 450502 £199.61
  1 Litre 450503 POR
Disposable SepFast Capture DEAE-500 column 5 x 1 ml 450504 £111.41
  1 x 5 ml 450505 £81.59
  1 x 10 ml 450506 £108.05
  1 x 20 ml 450507 £184.17
SepFast Capture DEAE-600 25 ml 450601 £71.72
  100 ml 450602 £199.61
  1 Litre 450603 POR
Disposable SepFast Capture DEAE-600 column 5 x 1 ml 450604 £111.41
  1 x 5 ml 450605 £81.59
  1 x 10 ml 450606 £108.05
  1 x 20 ml 450607 £184.17
SepFast Capture Q Products SepFast Capture DEAE Products SepFast Capture CM Products
Capture S Products
Description Quantity Code no. Price GBP
SepFast Capture S-400 25 ml 450701 £71.72
  100 ml 450702 £199.61
  1 Litre 450703 POR
Disposable SepFast Capture S-400 column 5 x 1 ml 450704 £111.41
  1 x 5 ml 450705 £81.59
  1 x 10 ml 450706 £108.05
  1 x 20 ml 450707 £184.17
SepFast Capture S-500 25 ml 450801 £71.72
  100 ml 450802 £199.61
  1 Litre 450803 POR
Disposable SepFast Capture S-500 column 5 x 1 ml 450804 £111.41
  1 x 5 ml 450805 £81.59
  1 x 10 ml 450806 £108.05
  1 x 20 ml 450807 £184.17
SepFast Capture S-600 25 ml 450901 £71.72
  100 ml 450902 £199.61
  1 Litre 450903 POR
Disposable SepFast Capture S-600 column 5 x 1 ml 450904 £111.41
  1 x 5 ml 450905 £81.59
  1 x 10 ml 450906 £108.05
  1 x 20 ml 450907 £184.17
SepFast Capture Q Products SepFast Capture DEAE Products SepFast Capture S Products
Capture CM Products
Description Quantity Code no. Price GBP
SepFast Capture CM-400 25 ml 451001 £71.72
  100 ml 451002 £199.61
  1 Litre 451003 POR
Disposable SepFast Capture CM-400 column 5 x 1 ml 451004 £111.41
  1 x 5 ml 451005 £81.59
  1 x 10 ml 451006 £108.05
  1 x 20 ml 451007 £184.17
SepFast Capture CM-500 25 ml 451101 £71.72
  100 ml 451102 £199.61
  1 Litre 451103 POR
Disposable SepFast Capture CM-500 column 5 x 1 ml 451104 £111.41
  1 x 5 ml 451105 £81.59
  1 x 10 ml 451106 £108.05
  1 x 20 ml 451107 £184.17
SepFast Capture CM-600 25 ml 451201 £71.72
  100 ml 451202 £199.61
  1 Litre 451203 POR
Disposable SepFast Capture CM-600 column 5 x 1 ml 451204 £111.41
  1 x 5 ml 451205 £81.59
  1 x 10 ml 451206 £108.05
  1 x 20 ml 451207 £184.17


>>Click here for SepFast Capture Q Products

>>Click here for SepFast Capture DEAE Products

>>Click here for SepFast Capture S Products

>>Click here for SepFast Capture CM Products

How to order

Products

Data Sheet

Data sheet