Glutathione SepFastTM MAG
Glutathione SepFast MAG is a magnetic affinity chromatography medium
used for the rapid one-step purification of Glutathione S-transferase (GST)-tagged
proteins with the aid of a magnet. Other Glutathione S-transferases and
Glutathione-binding proteins can also be purified with this adsorbent.
Reduced Glutathione ligand is immobilised to the carefully designed magnetic
support via very stable thiolether linkage.
GST-tagged proteins expressed in bacteria, yeasts, insects and mammalian cell
cultures can be readily purified in a single purification step. After binding of
target molecules, the magnetic resin can be readily isolated from the biological
feedstocks with the aid of a magnet. The GST tag can be cleaved in bound
condition or in eluted condition by specific proteases.
SepFast MAG is specially designed and fabricated for magnetic purification of
proteins in batch mode (i.e. stirred tank mode). The base matrix is made of
cross-linked agarose containing magnetic material. Therefore, the resin
possesses magnetic properties. Removal of liquid after each step, such as
binding, washing and elution can be readily done by fixing the resin with a
Its carefully controlled pore structure allows fast access of affinity ligands
by target protein molecules. Also, its high mechanical strength permits harsher
operational conditions. Clarified or unclarified cell lysates (for intracellular
proteins) or culture broths (for extracellular proteins) could be directly
processed with Glutathione SepFast MAG. All these translate into high process
flexibility and higher protein yield at shortened purification time.
Most commercial magnetic devices can be used to handle this resin; no special
magnet required. A magnetic stirring bar commonly available in laboratories can
be used to drag down the magnetic particles.
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Characteristics of Glutathione SepFast MAG
||Highly cross-linked 4% magnetic agarose
||50 – 150 μm
||>=20 μmol / ml resin
|Protein binding capacity
||Depends on the type of proteins and binding conditions; could be > 10 mg / ml resin
||Stable in all the commonly used aqueous buffers; stable at short contact to denaturants (e.g. 6M guanidine.HCl or 8M urea); stable to common clean-in-place agents e.g. 70% ethanol, 0.1 M NaOH, 0.1 M HCl.
||20% ethanol at 4oC – 8oC
*Other particle size available on request.