Glutathione SepFastTM MAG

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Introduction


Glutathione SepFast MAG is a magnetic affinity chromatography medium used for the rapid one-step purification of Glutathione S-transferase (GST)-tagged proteins with the aid of a magnet. Other Glutathione S-transferases and Glutathione-binding proteins can also be purified with this adsorbent.

Reduced Glutathione ligand is immobilised to the carefully designed magnetic support via very stable thiolether linkage.

GST-tagged proteins expressed in bacteria, yeasts, insects and mammalian cell cultures can be readily purified in a single purification step. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet. The GST tag can be cleaved in bound condition or in eluted condition by specific proteases.

SepFast MAG is specially designed and fabricated for magnetic purification of proteins in batch mode (i.e. stirred tank mode). The base matrix is made of cross-linked agarose containing magnetic material. Therefore, the resin possesses magnetic properties. Removal of liquid after each step, such as binding, washing and elution can be readily done by fixing the resin with a magnet.

Its carefully controlled pore structure allows fast access of affinity ligands by target protein molecules. Also, its high mechanical strength permits harsher operational conditions. Clarified or unclarified cell lysates (for intracellular proteins) or culture broths (for extracellular proteins) could be directly processed with Glutathione SepFast MAG. All these translate into high process flexibility and higher protein yield at shortened purification time.

Most commercial magnetic devices can be used to handle this resin; no special magnet required. A magnetic stirring bar commonly available in laboratories can be used to drag down the magnetic particles.
 

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Characteristics of Glutathione SepFast MAG

 
Base matrix Highly cross-linked 4% magnetic agarose
Particle size* 50 – 150 μm
Ligand Glutathione
Ligand density >=20 μmol / ml resin
Protein binding capacity Depends on the type of proteins and binding conditions; could be > 10 mg / ml resin
Chemical stability Stable in all the commonly used aqueous buffers; stable at short contact to denaturants (e.g. 6M guanidine.HCl or 8M urea); stable to common clean-in-place agents e.g. 70% ethanol, 0.1 M NaOH, 0.1 M HCl.
pH stability 4-12
Storage condition 20% ethanol at 4oC – 8oC

*Other particle size available on request.

Ordering information

 
Product Quantity Code no. Price GBP
Glutathione SepFast MAG 1 ml 320201-1ML 52.48
  5 ml 320201-5ML 135.61
  25 ml 320201-25ML POR

How to order

Operation Manual

Operation Manual