Glutathione SepFastTM Resins and Columns
Introduction
Glutathione SepFast is an affinity chromatography medium used for the
rapid one-step purification of Glutathione S-transferase (GST)-tagged proteins.
Other Glutathione S-transferases and Glutathione-binding proteins can also be
purified with this adsorbent.
Reduced Glutathione ligand is immobilised to the carefully designed porous
support via very stable thiolether linkage.
GST-tagged proteins expressed in bacteria, yeasts, insects and mammalian cell
cultures can be readily purified in a single purification step. GST tag can be
cleaved in bound condition or in eluted condition by specific proteases.
Glutathione SepFast adsorbents are specifically designed and fabricated for the
purification of GST-tagged proteins in batch (stirred tank), gravity flow or
packed column modes. Glutathione SepFast is made of highly cross-linked agarose
beads. Its carefully controlled pore structure allows fast access of affinity
ligands by target protein molecules. Also, its high mechanical strength permits
liquid to pass through gravity columns or packed columns at excellent flow
rates. Clarified or unclarified cell lysates (for intracellular proteins) or
culture broths (for extracellular proteins) could be directly processed with
Glutathione SepFast. All these translate into high process flexibility and
higher protein yield at shortened purification time.
Glutathione SepFast BG is supplied as loose resin or is supplied as pre-packed
columns.
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Characteristics of Glutathione SepFast resins and columns
Particle size |
50 – 150 μm |
Base matrix |
Highly cross-linked 4% agarose |
Ligand |
Glutathione |
Ligand density |
>=20 μmol / ml resin |
Protein binding capacity |
Depends on the type of proteins and binding conditions; could be > 10 mg / ml resin |
Chemical stability |
Stable in all the commonly used aqueous buffers; stable at short contact to denaturants (e.g. 6M guanidine.HCl or 8M urea); stable to common clean-in-place agents e.g. 70% ethanol, 0.1 M NaOH, 0.1 M HCl. |
pH stability |
3-12 |
Storage condition |
20% ethanol at 4oC – 8oC |
Ordering information
Product |
Quantity |
Code no. |
Price GBP |
Glutathione SepFast |
5 ml |
320101-5ML |
£114.11 |
|
10 ml |
320101-10ML |
£207.49 |
|
50 ml |
320101-50ML |
£677.27 |
|
1 Litre |
320101-1L |
POR |
Pre-packed Glutathione SepFast
column |
1 x 1ml |
320102-1x1ML |
£55.46 |
|
1 x 5 ml |
320102-1x5ML |
£169.24 |
|
1 x 10 ml |
320102-1x10ML |
POR |
|
1 x 20 ml |
320102-1x20ML |
POR |