Protein A SepFastTM MAG
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Introduction
Protein A SepFast MAG is an affinity chromatography medium possessing magnetic properties. It is employed for the purification of immunoglobulins using magnets. The adsorbent particle can be easily settled down with the aid of a magnet. Its purification power has been well documented in various antibody purification applications, such as isolation and purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
Protein A is immobilised to highly porous and highly cross-linked agarose incorporating magnetic material. Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
Protein A SepFast MAG can be used in any commercially available magnetic device or be settled with any magnet (such as a magnet bar or magnetic plate etc).
- Key benefits:
- Tailored base matrix design for batch magnetic purification of immunoglobulins
- Simple and convenient operation, no chromatography training required
- Flexible operational conditions
- Low cost
- No special magnet required
- Full operation manual (including purification conditions) supplied
Characteristics of Protein A SepFast MAG
Matrix | Cross-linked agarose with magnetic material |
Binding capacity | Approx. 15 mg human IgG / ml resin |
Chemical compatibility | The commonly used reagents for antibody purifications |
Storage condition | 4oC – 8oC |
Ordering information
Product | Quantity | Code no. | Price GBP |
Protein A SepFast MAG | 500 μl | 280101 | £ 70.04 |
Protein A SepFast MAG | 5 ml | 280102 | POR |