The base matrix is reacted with cyanogen bromide (CNBr) to create active groups that react with the primary amine groups in the molecules to be immobilized.

CNBr-activated agarose beads have a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to CNBr-activated agarose is a widely used, successful, and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.

CNBr activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particle has an open pore structure with excellent mass transfer properties to large protein molecules.

Our pre-activated SepFast beads can be readily employed to make various affinity chromatography media for both small scale and large scale purification applications. The application areas cover immobilization of proteins, peptides and nucleic acids.

Coupling manual is supplied with each product.